Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: MRMP mediates upregulation of MPC1. (A) MPC1 mRNA expression in MRMP-infected HCAECs was determined using qRT-PCR ( n = 3). (B,C) MPC1 protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Expressing, Infection, Quantitative RT-PCR, Western Blot

Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: UK5099 alleviates MRMP-induced mitochondrial damage (A) mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey’s post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: UK5099 alleviates MRMP-induced pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay. (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces mitochondrial damage. (A) Mitochondrial morphology was imaged using TEM ( n = 3). Scale bar: 500 nm. (B) Quantification of the average individual mitochondrial area ( n = 3). (C,D) mRNA levels in MRMP-infected HCAECs were determined using qRT-PCR ( n = 3). (E,F) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Infection, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: MG132-mediated PINK1 deficiency induces pyroptosis of HCAECs. (A,B) Cytokine release was determined using ELISA ( n = 3). (C) Cell viability was determined using CCK-8 assay ( n = 3). (D) Cytotoxicity was determined using LDH assay ( n = 3). (E,F) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (G–J) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Expressing, Infection, Western Blot

Journal: Frontiers in Pediatrics

Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy

doi: 10.3389/fped.2026.1731155

Figure Lengend Snippet: MPC1 induces pyroptosis of HCAECs via inhibiting PINK1-dependent mitophagy. (A–D) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). (E,F) Cytokine release was determined using ELISA ( n = 3). (G) Cell viability was determined using CCK-8 assay ( n = 3). (H) Cytotoxicity was determined using LDH assay ( n = 3). (I,J) Cell death was determined using TUNEL assay ( n = 3). Scale bar: 50 μm. (K–N) Protein expression in MRMP-infected HCAECs was determined using Western blot ( n = 3). ** p < 0.01, one-way ANOVA followed by Tukey's post hoc test.

Article Snippet: Human coronary endothelial cells (HCAECs) were purchased from ATCC, USA.

Techniques: Expressing, Infection, Western Blot, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay

Journal: iScience

Article Title: Comparative effects of hemodialysis modalities on microparticle induction and neutrophil activation in a randomized cross-over study

doi: 10.1016/j.isci.2025.113485

Figure Lengend Snippet: Effects of post-hemodialysis microparticles from the mixed dilution online-HDF (OL-HDF) or hemodialysis with median cut-off dialyzer (MCO-HDX) on human coronary artery endothelial cells (HCAECs) The representation of carboxyfluorescein succinimidyl ester (CSFE)-stained MPs (green color) represented by CSFE fluorescent intensity with the representative immunofluorescent pictures (A and B) are demonstrated ( n = 6/group, scale bar = 10 μ m). The red color in A is zona occluden-1 (ZO-1; a tight junction molecule) and the blue color is DNA-stained by 4′,6-diamidino-2-phenylindole (DAPI). The influences of the post-dialysis MPs from OL-HDF (C) or MCO+HDX (D) with 1x10 4 (light color) or 1x10 5 (heavy color) particles/μL at 24 h after inoculation, compared with control (only media but not MPs) in the condition with or without phorbol myristate acetate (PMA, the positive control activator) on HCAECs as indicated by vascular cell adhesion molecule 1 (VCAM-1) and cell apoptosis, are shown. The effect of MPs on tight junction molecules ZO-1 is also demonstrated through the intensity score of the red color with representative immunofluorescent (E, F) ( n = 6/group, scale bar = 10 μ m). Notably, the MPs were not stained to avoid the noise from the green-fluorescent color. ∗ p < 0.05 vs. control within group; # p < 0.05 vs. same condition between groups.

Article Snippet: Primary Human Coronary Endothelial Cells (HCAECs), Female , ATCC , PCS-100-020 Lot No. 70001400.

Techniques: Staining, Control, Positive Control

Journal: bioRxiv

Article Title: Chromatin landscape and enhancer-gene interaction differences between three cardiac cell types

doi: 10.1101/2025.09.23.678180

Figure Lengend Snippet:

Article Snippet: Human coronary artery smooth muscle cells (HCASMC) and Human coronary artery endothelial cells (HCAEC) were all commercially bought from Thermo Fisher Scientific, Waltham, MA, U.S.A. (product code: C-017-5C) and the American Type Culture Collection (ATCC), Manassas, VA, U.S.A. (Product code: PCS-100-020), respectively.

Techniques: Modification

Journal: International journal of molecular sciences

Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.

doi: 10.3390/ijms26083732

Figure Lengend Snippet: Figure 3. Volcano plot of genes that qualified as differentially expressed (DEGs) between UWOI and UWI groups in HCAECs. Blue dots in the upper left quadrant represent down-regulated genes, red dots in the upper right quadrant represent up-regulated genes, and black dots present stable genes (p < 0.05).

Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques:

Journal: International journal of molecular sciences

Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.

doi: 10.3390/ijms26083732

Figure Lengend Snippet: Figure 4. Hierarchical cluster. DEG heatmap of supervised analysis of 100 differentially expressed genes between UWOI (orange upper bar) and UWI (dark green upper bar) groups in HCAECs. The upper left quadrant shows the color key and histogram representing the behavior of DEGs for the UWOI and UWI groups in the HCAEC model. The samples are in the columns, and the genes are in the rows. Red color represent up regulated genes, and blue color represent down-regulated genes with different expression intensity.

Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: Gene Expression Profile of Cultured Human Coronary Arterial Endothelial Cells Exposed to Serum from Chronic Kidney Disease Patients: Role of MAPK Signaling Pathway.

doi: 10.3390/ijms26083732

Figure Lengend Snippet: Figure 6. Molecular networks of HCAECs exposed to uremic serum. Associated genes derived from String were based on correlations in order to characterize cellular and molecular functions and identify enriched canonical pathways/networks for the list of selected candidate genes, according to Gen Ontology data. The solid lines represent the interactions between genes, and the nodes (spheres) represent the proteins that are associated with the respective genes. Each color of the nodes represents evidence of protein–protein interaction. Pink indicates experimentally determined/post-translational modifications; blue indicates gene co-occurrence; green indicates gene neighborhood; black indicates co-expression; red indicates gene fusion. Nodes with ribbon-like structures represent the availability of 3D structural information of the protein being predicted.

Article Snippet: Human coronary arterial endothelial cells (HCAECs) (PCS-100-020) and Endothelial Cell Enrichment Kit-VEGF medium (PCS-100-041) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Derivative Assay, Expressing